Figure 6

Expression of TsNd at different developmental stages and immunolocalization in T. spiralis . A-H: The results of IFT with whole parasite of T. spiralis different stages reacted with anti-rTsNd serum. The notable green fluorescent staining is found on the surface and in the body of ML at 42 dpi (A), IIL at 6 hpi (B), female AW at 3 dpi (C), male AW at 3 dpi (D) and NBL (E).The ML with mouse infection serum (F) as a positive control; ML did not show recognition by normal mouse serum (G) and PBS (H) as a negative control. I–N: The results of IFT with the sections of whole worms (ML at 42 dpi, IIL at 6 hpi and AW 3 dpi) reacted with anti-rTsNd serum. The immunostaning is seen at the cuticle, stichocytes and genital primordium of ML (I), IIL (J) and AW (K). The ML reacted with mouse infection serum (L) as a positive control; ML did not show recognition by normal mouse serum (M) and PBS (N) as a negative control.