Table 1 The conditions of serpin inhibition reactions against commercially available bovine, porcine and human serine proteases

Chymotrypsin

50 mM Tris-HCl, 150 mM NaCl, 20 mM CaCl₂, 0.01 % Triton X-100, pH 8.0

N-Succinyl-Ala-Ala-Pro-Phe-p-nitroanilide

Elastase

50 mM Hepes, 100 mM Nacl, 0.01 % Triton X-100, pH 7.4

N-Succinyl-Ala-Ala-Ala-p-nitroanilide

Kallikrein

20 mM Tris-HCl, 150 mM NaCl, 0.02 % Triton X-100, pH 8.5

N-Benzoyl-Pro-Phe-Arg-p-nitroanilide hydrochloride

Plasmin

20 mM Tris-HCl, 150 mM NaCl, 0.02 % Triton X-100, pH 8.5

Gly-Arg-p-nitroanilide dihydrochloride

Thrombin

50 mM Tris-HCl, 150 mM NaCl, 20 mM CaCl₂, 0.01 % Triton X-100, pH 8.0

Sar-Pro-Arg-p-nitroanilide dihydrochloride

Trypsin

50 mM Tris-HCl, 150 mM NaCl, 20 mM CaCl₂, 0.01 % Triton X-100, pH 8.0,

N-Benzoyl-Phe-Val-Arg-p-nitroanilide hydrochloride