Figure 5

Expression of Tspst at different developmental stages and immunolocalization in T. spiralis. A–H: The results of IFT with whole parasite of T. spiralis different developmental stages reacted with anti-rTspst serum. The notable immunostaning is found in the entire bodies of IIL at 2 hpi (A), AW at 3 dpi (B), AW at 7 dpi (C), NBL (D) and ML at 42 dpi (E). The ML at 42 dpi reacted with infection serum (F) as a positive control; ML at 42 dpi did not show recognition by PBS (G) and normal serum (H) as a negative control. I–P: The results of IFT with the sections of intestines and skeletal muscles of infected mice reacted with anti-rTspst serum. The immunostaning is seen at the cuticle and internal organs of IIL at 2 hpi (I), AW at 3 dpi (J), AW at 7 dpi (K), PEL at 19 dpi (L) and ML at 42 dpi (M). The ML at 42 dpi reacted with infection serum (N) as a positive control; ML at 42 dpi did not show recognition by PBS (O) and normal serum (P) as a negative control.