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Figure 1 | Parasites & Vectors

Figure 1

From: Leishmania enriettii: biochemical characterisation of lipophosphoglycans (LPGs) and glycoinositolphospholipids (GIPLs) and infectivity to Cavia porcellus

Figure 1

Experimental procedures scheme. Promastigotes of L. enriettii were used for infection in C. porcellus in the presence/absence of Salivary Gland Extract (SGE). LPG and GIPLs were extracted with organic solvents and purified using Phenyl-Sepharose. LPG purification was confirmed by western-blot. The LPG was depolymerased using mild acid hydrolysis (0.02 N HCl, 5 min, 100°C) and the repeat units were dephosphorylated using alkaline phosphatase. The profiles were analysed by Fluorophore-assisted carbohydrate electrophoresis (FACE). Purified GIPLs were subjected to strong acid hydrolysis (2 N trifluoroacetic acid, 100°C, 3 hours) and monosaccharides were analyzed by FACE. Purified LPGs and GIPLs were incubated with murine peritoneal macrophages, CHO cells and J774.A1 cells for NO, cytokines, MAPKs and NF-kB activation.

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Parasites & Vectors

ISSN: 1756-3305

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