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Table 2 Lymphocyte proliferation and cytokine production by splenocytes stimulated with peptides

From: Protective immunity induced by peptides of AMA1, RON2 and RON4 containing T-and B-cell epitopes via an intranasal route against toxoplasmosis in mice

Groups #

Lymphocyte SI ##

Cytokine production (pg/ml) ##

IFN-γ

IL-2

IL-4

IL-10

PBS

0.76 ± 0.04 a

54.96 ± 12.07 a

57.17 ± 8.64 a

67.17 ± 6.43 a

84.13 ± 6.43

AMA1

2.04 ± 0.12 c

103.09 ± 18.99 b

156.58 ± 12.47 b

147.75 ± 11.67 b

102.25 ± 14.67

RON2

2.14 ± 0.24 c

97.39 ± 13.21 b

147.33 ± 11.92 b

136.42 ± 19.22 b

100.55 ± 19.22

RON4

2.21 ± 0.19 c

94.53 ± 16.60 b

134.42 ± 16.30 b

145.58 ± 12.98 b

97.75 ± 12.98

A1 + R2

3.17 ± 0.35 c

156.62 ± 17.25 c

219.71 ± 17.11 c

190.17 ± 21.11 c

110.13 ± 11.11

A1 + R4

2.21 ± 0.31 c

116.52 ± 16.60 b

183.84 ± 14.62 c

172.08 ± 16.22 b

111.51 ± 16.22

R2 + R4,

2.24 ± 0.40 c

123.36 ± 16.21 b

165.75 ± 24.20 b

174.83 ± 41.98 b

110.81 ± 11.98

A1 + R2 + R4

2.54 ± 0.32 c

108.52 ± 21.21 b

162.34 ± 17.11 b

174.17 ± 21.11 b

109.75 ± 11.11

  1. # n = 6 per group.
  2. ##Splenocytes from mice were harvested 2 weeks after the last immunization. SI represents stimulation index, calculated as the ratio of the OD450 of stimulated cells to the OD450 of unstimulated cells. The values for IFN-γ were obtained at 96 h, while the values for IL-2 and IL-4 were obtained at 24 h, and the values for IL-10 were obtained at 72 h. The results are presented as the mean ± SD of three replicate experiments. a vs b: P < 0.05; b vs c: P < 0.05; a vs c: P < 0.01.
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Parasites & Vectors

ISSN: 1756-3305

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