Figure 3

PCR amplification and alignment for Leishmania spp. in mummy A74. (A) PCR amplification fragment of a 120 bp fragment of the kinetoplastid mitochondrial DNA of Leishmania spp. obtained using Degrave et al. (1994) primers [35] (Lane 1: molecular marker 50 bp; lane 2: Tissues from the abdominal cavity; lane 3: Tissues from the abdominal region; lane 4: negative control; lane 5: Tissue adhering to the 10th rib; lane 6: Cortical bone from the 10th rib; lane 7: Bone marrow from the 10th rib). (B) Alignment for Leishmania tarentolae in mummy A74 (without primers) (1. Reference sequence for L. tarentolae from a culture available in Genbank: HM579788); 2. L. tarentolae aDNA sequence detected in mummy A74; 3. Leishmania infantum aDNA from Eleonora di Toledo’s skeletal remains [42] used for comparison; 4. mDNA of L. infantum available in GenBank: AB678348 used for comparison; 5. DNA of L. major available in GenBank: EU370908 used for comparison; 6. DNA of L. donovani available in GenBank: L19877 used for comparison.