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Table 1 Detail of the biopsies taken from mummy A74 and submitted to paleomolecular investigations, the primers used and the results obtained

From: Leishmania tarentolae molecular signatures in a 300 hundred-years-old human Brazilian mummy

Type of biopsies

Primers used

Results

Fragment of tissues adhering to the 10th rib (Figure 1E)

13A: 5â€Č GGGGAGGGGCGTTCTGCGAA 3â€Č

+

13B: 5â€Č SSSCCMCTATWTTACACCAACCCC 3â€Č [36]

13A: 5â€Č GTGGGGGAGGGGCGTTCT 3â€Č

-

13B: 5â€Č ATTTTACACCAACCCCCAG 3â€Č [37]

Fragments of tissues from abdominal region (Figure 1D1)

13A: 5â€Č GGGGAGGGGCGTTCTGCGAA 3â€Č

+

13B: 5â€Č SSSCCMCTATWTTACACCAACCCC 3â€Č [36]

13A: 5â€Č GTGGGGGAGGGGCGTTCT 3â€Č

+

13B: 5â€Č ATTTTACACCAACCCCCAG 3â€Č [37]

Fragment of tissues from abdominal cavity (Figure 1D2)

13A: 5â€Č GGGGAGGGGCGTTCTGCGAA 3â€Č

+

13B: 5â€Č SSSCCMCTATWTTACACCAACCCC 3â€Č [36]*

13A: 5â€Č GTGGGGGAGGGGCGTTCT 3â€Č

-

13B: 5â€Č ATTTTACACCAACCCCCAG 3â€Č [37]

Cortical bone from the 10th rib (Figure 2A)

13A: 5â€Č GGGGAGGGGCGTTCTGCGAA 3â€Č

-

13B: 5â€Č SSSCCMCTATWTTACACCAACCCC 3â€Č [36]**

Bone marrow from the 10th rib (Figure 2B)

13A: 5â€Č GGGGAGGGGCGTTCTGCGAA 3â€Č

+

13B: 5â€Č SSSCCMCTATWTTACACCAACCCC 3â€Č [36]**

  1. * = Sample positive for Leishmania tarentolae in the first set of by using Rogers et al. [37] primers.
  2. ** = Samples from cortical bone and bone marrow analysed in a second round of analyses by using Degrave et al. [36] primers. With Degrave et al. (1994) primers, a more intense amplification of the PCR products was observed.