Figure 4

Ultrastructural cytochemistry of Litomosoides chagasfilhoi by OsKI staining. A – General view of the body wall showing the cuticle (C) and hypoderm (H) that present the endosymbionts (E). There is a positive reaction specific to regions near the bacteria (thin arrow) (Bar 20 μm). B – Note the electron-dense and reticular structures close to the endosymbiont (thin arrow) (Bar 20 μm). C – Detail showing the intimate association between the bacterial membrane and the electron-dense structures that resemble the membranes of the endoplasmic reticulum (thin arrow) (Bar 10 μm).