Real-time quantitative PCR analysis of Ts-clp. After reverse transcription, the cDNA of ML, L6h, L24h, Ad2, Ad3, Ad5, and NBL was used as a template for real-time quantitative PCR using SYBR Green. Each reaction was performed in triplicate on the plate, and the experiment was repeated three times. All the fold changes were relative to the NBL stage. *Data are significantly different from NBL (P < 0.05).