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Archived Comments for: Silencing the buzz: a new approach to population suppression of mosquitoes by feeding larvae double-stranded RNAs

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  1. Reproducability of bacteria feeding experiments

    Steve Joyce, Oxitec Ltd.

    19 June 2015

    To the Authors,

    As it stands, the methodology concerning the feeding of dsRNA expressing bacteria to larvae contains insufficient information for independent replication of some of the experiments in this article. Specifically, the volume of the bacterial culture centrifuged, and the volume of 1% LB agar used to suspend the pellet are not stated in the section entitled “Introducing dsRNA to mosquito larvae”. 

    Please could you provide these details so that the results may be replicated?

    Many Thanks

    Competing interests

    Oxitec Ltd. is a pioneer in novel insect control.

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