Clonorchis sinensis acetoacetyl-CoA thiolase: identification and characterization of its potential role in surviving in the bile duct

Table 1 Enzymatic activity of Cs ACAT in adult worms incubated with different concentrations of lecithin

Fraction	Concentration (μg/μl)	Volume (μl)	Total protein (μg)	Total activity units (μmol/min)	Specific activity units/mg protein	Relative activity (%)
Naïve ACAT activity#	6.68	5	33.41	0.024	0.729	100
6 mM lecithin*	9.82	5	49.09	0.014	0.293	40.19
0.6 mM lecithin*	11.03	5	55.15	0.01	0.181	24.81
0.06 mM lecithin*	12.99	5	64.95	0.011	0.168	23.03
Control**	11.12	5	55.58	0.006	0.104	14.31

The crude extracts of worms incubated with different conditions were collected at 48 h. #, Enzymatic activity of CsACAT in worms newly recovered from liver of infected rat was set as 100%; *, Lecithin was firstly dissolved with 100% alcohol in a 56°C water bath and then diluted with PBS to prepare the mixture containing different concentrations of lecithin; **, Worms cultured with 0.6% alcohol in PBS was applied as a negative control.

ISSN: 1756-3305