Long-term experiment showing bacteria population in
midgut infected with
. CFU counts were made with R. prolixus 5th instar nymphs 12 days after feeding (DAF) on blood without parasites. Previously, 4th instar nymphs were fed on inactivated blood with or without 1 × 106 epimastigotes/mL. Bars represent mean ± SEM of three independent experiments. Each point represents the CFU from three pools of three insects (n = 9). Means were compared using Student T-test or Mann–Whitney test; **p < 0.01 and *p < 0.05.