Figure 2

Conserved and antigenic tick cystatin regions for JpIocys2a peptide selection. Antigenic index plots for tick cystatins were predicted using the Jameson–Wolf algorithm. Graphic increased positivity shows predictive antigenic sites. Alignment shows conserved regions between JpIocys2a and R. microplus cystatins. Black boxes indicate conserved and antigenic amino acid region for each sequence. Asterisk indicate the selected region for peptide synthesis.