Figure 1

PCR amplification, nucleotide and deduced amino acid sequence of O. turicata subolesin gene. A) PCR amplification of subolesin gene is shown. Band of approximately 470 bp was evident in both unfed and fed O. turicata ticks. The 28S rRNA levels were shown as controls. Arrow indicates subolesin PCR fragments. B) Percent identity (horizontally above black boxed diagonal) and divergence (vertically below black boxed diagonal) of O. turicata subolesin nucleotide sequence in comparison to O. erraticus and O. moubata subolesin sequences is shown. C) The nucleotide sequence of O. turicata subolesin gene was obtained by sequencing PCR products cloned in the pGEMT-easy vector. The deduced amino acid sequence is shown as a single letter code below the nucleotide sequence.