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Table 2 Comparison of Culicoides COI barcode sequences of three Palaearctic subgenera; Avaritia , Culicoides and Monoculicoides

From: Delineation of Culicoides species by morphology and barcode exemplified by three new species of the subgenus Culicoides (Diptera: Ceratopogonidae) from Scandinavia

Species   SNP-sites Var Div Comments
  N N % %  
Subgenus: Avaritia      
C. chiopterus 32 31 2.3 18  
C. dewulfi 53 11 0.85 21  
C. imicola 80 45 2.8 18  
C. obsoletus 127 42 3.8 17  
C. scoticus 89 24 1.7 17  
Subgenus: Culicoides      
C. deltus 12 5 0.85 17  
C. fagineus F1 3 2 0.42 19 C. fagineus F1 and F2 diverge 8.5% from each other.
C. fagineus F2 4 7 1.5 19  
C. flavipulicaris 2 1 0.21 17  
C. grisescens 22 10 1.3 17 C. grisescens G1 and G2 diverge 11% from each other.
C. grisescens G2 6 12 1.9 17  
C. impunctatus 20 7 0.64 18  
C. lupicaris 15 9 0.42 16  
C. lupicaris L2 8 3 0.42 15 C. lupicaris L2 diverge 14% from C. lupicaris.
C. newsteadi 9 1 0.21 16  
C. newsteadi N1 1 n.a. n.a. 16 C. newsteadi N1 diverge 14-17% from C. newsteadi, N2, N3, N4 and N5.
C. newsteadi N2 5 8 1.5 17 C. newsteadi N2 diverge 14-16% from N3, N4 and N5.
C. newsteadi N3 ( C. halophilus ) 17 5 0.64 19 C. newsteadi N3 diverge 14-16% from C. newsteadi, N4 and N5.
C. newsteadi N4 ( kalix ) 10 0 0.00 13 C. newsteadi N4 diverge 14% and 5.9% from N4 and N5, respectively.
C. newsteadi N5 (dk3) 5 6 1.3 15 C. newsteadi N5 and C. newsteadi diverge 15% from each other.
C. pulicaris 57 16 0.63 17 C. pulicaris P3 diverge 10% from C. pulicaris.
C. pulicaris P3 2 1 0.21 15  
Culicoides dk1 9 0 0.00 18 Culicoides dk1 diverge 17-18% from C. pulicaris and P3, respectively.
CH 12 23 4.6 15 Culicoides CH diverge 8-9% from C. pulicaris and P3
C. punctatus 61 32 0.63 0  
C. subfagineus 3 2 0.42 17  
Subgenus: Monoculicoides      
C. nubeculosus 12 3 0.64 19  
C. puncticollis 20 0 0.00 19  
C. riethi 16 1 0.21 19  
C. stigma 6 0 0.00 17  
  1. Species are grouped according to their subgenus status; species names are those used in the papers where the sequences are published. N: Number of Culicoides sequences which are >95% homologous. SNP-Sites: Number of variable sites in the 472 bp COI barcode region. Var: The highest intraspecific difference observed in pairwise comparisons. Div: Highest divergence from type-species C. punctatus (GenBank AM236733) observed in pairwise comparisons. n.a.: not applicable.