|
Mice sacrificed 8 weeks p.i.
|
Mice sacrificed 15 weeks p.i.
|
---|
|
#3
|
#4
|
#6
|
#8
|
#10
|
#1
|
#2
|
#5
|
#7
|
#9
|
---|
Inoculated ear (IE)
|
++
|
+++
|
++++
|
++++
|
neg.
|
+
|
++
|
+++
|
++++
|
++++
|
Contralateral ear (CE)
|
neg.
|
neg.
|
neg.
|
neg.
|
neg.
|
neg.
|
neg.
|
neg.
|
+
|
++
|
Draining lymph node of the IE
|
+++
|
+++
|
++++
|
++++
|
neg.
|
neg.
|
neg.
|
++++
|
+++++
|
+++++
|
Draining lymph node of the CE
|
neg.
|
neg.
|
neg.
|
neg.
|
neg.
|
neg.
|
neg.
|
neg.
|
neg.
|
neg.
|
Paws
|
neg.
|
neg.
|
neg.
|
neg.
|
neg.
|
neg.
|
neg.
|
neg.
|
neg.
|
neg.
|
Tail
|
neg.
|
neg.
|
neg.
|
neg.
|
neg.
|
neg.
|
neg.
|
neg.
|
neg.
|
neg.
|
Spleen*
|
+
|
++
|
+
|
++
|
+++
|
neg.
|
neg.
|
++
|
++
|
++
|
Liver
|
neg.
|
neg.
|
neg.
|
+
|
neg.
|
neg.
|
neg.
|
+
|
++
|
++
|
Blood
|
n.d.
|
n.d.
|
neg.
|
neg.
|
++
|
n.d.
|
n.d
|
neg.
|
neg.
|
neg.
|
- *Spleens of mice 5, 7 and 9 were positive also by parasite cultivation; in other mice cultivation attempts were not done.
- Quantity of parasites was tested by qPCR and scored as + (<102), ++ (102-103), +++ (103-104), ++++ (104-105), +++++ (>105).
- neg., negative, n.d., not done; p.i., post infection.