Elaboration of anti-promastigote efficacy of
fractions. (a). IC50 determination against L. donovani promastigotes. L. donovani promastigotes (2 × 106 ml−1) were incubated with different concentrations of ALE, ASE and pentamidine as described in Methods. Each point corresponds to the mean ± SE of samples in triplicate and data is from one of three independent experiments. (b). Light microscopy analysis of promastigote morphology. Promastigote forms of L. donovani were treated with ALE, ASE and pentamidine (500 μg ml−1) and stained with erythrosin B. Photomicrographs were recorded at 100 X under oil immersion.