Cell cycle analysis by PI staining.
L. donovani promastigotes (2 × 106 ml−1) were incubated with ALE, ASE and pentamidine (500 μg ml−1). After 72 h, the samples were processed for cell cycle as described in Methods. The bar diagram depicts the percentage of cells in the sub G0-G1 phase. ***P < 0.001 with respect to parasite control.