Apoptosis mediated leishmanicidal activity of Azadirachta indica bioactive fractions is accompanied by Th1 immunostimulatory potential and therapeutic cure in vivo

Table 2 Bioactivity of ALE and ASE against L. donovani promastigotes and amastigotes as well as RAW 264.7 macrophages

*A. indica* fractions/compounds	IC ₅₀ (μg ml ⁻¹ ) promastigotes	IC ₅₀ (μg ml ⁻¹ ) amastigotes	CC ₅₀ (μg ml ⁻¹ ) macrophages	SI
ALE	34 ± 0.81	17.66 ± 2.88	461 ± 7.07	26.10
ASE	77.66 ± 3.13	24.66 ± 4.98	>500	-
Pentamidine	1.4 ± 0.249	0.87 ± 0.17	45.0 ± 2.80	51.72

Values represent Mean ± SE, SI = Selectivity Index (calculated as described in Methods), “-” indicates that SI could not be determined.

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