Cell proliferation assay performed by the crystal violet technique measuring cell viability over a 10 day period. The experiment was carried out in canine endothelial (A) and smooth muscle cells (B) untreated or treated with 1 μg/ml of DiES + 10 μg/ml of PLG, 1 μg/ml of DiES, 10 μg/ml of PLG, or with 1 μg/ml of DiES + 10 μg/ml of PLG + 50 mM of the εACA. Results are expressed as number of viable cells (x 10,000). Each point is the mean ± SD from three independent experiments. The asterisk (*) designates significant (p < 0.05) differences between DiES + PLG treatment and control groups.