Skip to main content

Table 6 Diagnostic test performance of MT PCR and HRM analysis

From: Comparison of the performance of three PCR assays for the detection and differentiation of Theileria orientalis genotypes

Outcome Median (95% PI) a
MT PCR  
Diagnostic sensitivity (%) 98.9 (97.0, 99.8)
Diagnostic specificity (%) 97.8 (94.5, 99.3)
PCR-HRM analysis  
Diagnostic sensitivity (%) 97.6 (95.6, 99.0)
Diagnostic specificity (%) 96.5 (94.3, 98.3)
Estimated true prevalence
Group 1 (%) 98.2 (95.5, 99.6)
Group 2 (%) 0 (0, 2.14)
Agreement between tests  
PABAK in group 1 95.2 (90.2, 98.0)
PABAK in group 2 94.0 (87.3, 97.9)
Conditional dependence between tests b
ρ+ 0.301 (0.006, 0.715)
ρ- 0.480 (0.106, 0.772)
Model fit  
pD 12.8
DIC 35.1
  1. The diagnostic sensitivity of the MT PCR was defined based on a cut-off of ≥ 1 DNA copies.
  2. aPI, probability interval.
  3. b ρ+ is the conditional correlation between MT PCR and PCR-HRM outcomes for infected animals, and ρ- is the conditional correlation for uninfected animals.
  4. PABAK = prevalence-adjusted bias-adjusted Cohen’s kappa (ĸ) statistic.
  5. pD = effective dimension (model complexity).
  6. DIC = Deviance information criterion, a generalisation of Akaike’s Information Criterion (AIC).