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Figure 4 | Parasites & Vectors

Figure 4

From: Transmembrane protein 63A is a partner protein of Haemonchus contortus galectin in the regulation of goat peripheral blood mononuclear cells

Figure 4

Knockdown of the TMEM63A gene and rHco-gal-m-treatment affected the PBMC proliferation. PBMC can be share as blank group (group 1), ns siRNA group (group 2), ns siRNA/g group (group 3), 63A siRNA group (group 4) and 63A-siRNA/g group (group 5), which were incubated with equal volume reduced serum medium (group 1), ns siRNA (group 2 and 3) and TMEM63A-siRNA-1 (group 4 and 5) for 60 h. RHco-gal-m in all RNA interference groups (group 3 and 5) was added 12 h before the end of RNA interference period. The proliferation was measured by CCK-8 incorporation. Cells proliferation index was calculated considering the OD450 values in blank group as 100%. Results presented here were collected from one independent experiment and were representative of three independent experiments. Data were represented as mean ± SD, n = 6; *p < 0.001 versus the ns siRNA group; an asterisk and a capped line designates two groups differ significantly (p < 0.001).

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Parasites & Vectors

ISSN: 1756-3305

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