Figure 7
Knockdown of the TMEM63A gene and rHco-gal-m-treatment affected the PBMC migration. PBMC can be share as blank group (group 1), ns siRNA group (group 2), ns siRNA/g group (group 3), 63A siRNA group (group 4) and 63A siRNA/g group (group 5), which were incubated with equal volume reduced serum medium (group 1), ns siRNA (group 2 and 3) and TMEM63A-siRNA-1 (group 4 and 5) for 60 h. RHco-gal-m in all RNA interference groups (group 3 and 5) was added 12 h before the end of RNA interference period. Then the random migration was determined. The data are presented asbox-and-whiskers blot, with the box containing 50% of the values, and the whiskers showing the highest and the lowest values. The median is indicated by the horizontal bar, the mean value as square. Results presented here were collected from one independent experiment (n = 4) and were representative of three independent experiments; *p < 0.001 versus the ns siRNA group.