Figure 1

Phylogenetic tree of near-complete 18S rRNA gene sequences of T. annae , B. microti , and B. microti -related parasites using maximum likelihood. The sequence of each isolate is labeled with its gene accession number, isolate designation, host, and geographic origin of isolation. The bootstrap values based on 1,000 replicates are displayed next to the branches. The tree is rooted using closely related Babesia parasites infecting rodents and carnivores of Clade I as defined in Schnittger et al. [3]. Wherever applicable, the number of pooled sequences is given. Accession number of pooled sequences are Babesia rodhaini: M87565, DQ641423, AB049999; Babesia sp. leopard [50]: JQ861967, JQ861965, JQ861972; Babesia leo: AY452708, AF244911; Babesia felis: AY452698, AY452699, AY452700, AY452700, AY452701, AY452702, AY452703, AY452704, AY452705, AY452706, AY452707. Babesia sp. baboon: GQ225744 [51] and Babesia sp. caracal AF244913, AF244914 [52]. Clades marked by brackets display a highly significant bootstrap value (≥85). The evolutionary distance is shown in the units of the number of base substitutions per site.