Table 1 Primers targeting host cytochrome b gene
From: Identification of phlebotomine sand fly blood meals by real-time PCR
Host species | Primers | CG content | Tm (°C) | Product size (bp) |
|---|---|---|---|---|
Canis lupus familiaris | f5′ - AGCGCCGTCTAACATCTCTG - 3′ | 55.45 | 55.90 | 118 |
r5′ - TGTGGCTGTGTCCGATGTAT - 3′ | 50.89 | 59.10 |  | |
Equus caballus | f5′ - CAGCCAGTGGAACACCCATA- 3′ | 55.00 | 59.67 | 103 |
r5′ - TGTTTTCGATGGTGCTTGCG - 3′ | 50.00 | 60.04 |  | |
Felis catus | f5′ - AGAATGGATCTGAGGGGGCT - 3′ | 55.00 | 60.03 | 108 |
r5′ - AGGTGTACTGCTGCTAAGGC - 3′ | 55.00 | 59.75 |  | |
Gallus gallus | f5′ - CAGCAGACACATCCCTAGCC - 3′ | 60.00 | 60.18 | 104 |
r5′ - GAAGAATGAGGCGCCGTTTG - 3′ | 55.00 | 60.18 |  | |
Homo sapiens | f5′ - AGGCGTCCTTGCCCTATTAC- 3′ | 55.00 | 59.53 | 104 |
r5′ - GTGATTGGCTTAGTGGGCG - 3′ | 55.00 | 60.39 |  | |
Rattus rattus | f5′ - GAATTGGGGGCCAACCAGTA - 3′ | 55.00 | 59.00 | 109 |
r5′ - TCAATGATTCCGGAGATTGGT - 3′ | 42.86 | 57.00 |  |