Figure 1
Inhibitory effects on IgGs binding activities by egg antigens devoid of proteins. A and B. Higher levels of IgG against untreated egg antigens (SjEA) than against periodate-treated SjEA (pSjEA) were found in sera from Schistosoma japonicum infected mice (A) or infected humans (B). Data shown were mean ± SEM of OD values of variously diluted sera in infected mice (n = 5) or in infected patients (n = 7). Data on mice were representative of two separate experiments. C. SjEA devoid of proteins by proteinase K (PK) and carboxypeptidase Y (CPD Y) treatment was capable of inhibiting IgG bindings with SjEA whether in infected mice or infected humans at dose-dependent fashion. A representative competitive inhibition curve on IgGs in infected sera was shown on panel 1 (mouse) and panel 3 (human) using untreated SjEA as positive control for inhibition assay and OVA as negative control. A summary on calculated percentage of inhibition from infected mice (n = 5) or infected humans (n = 7) by proteinases-treated SjEA was shown at panel 2 and panel 4. D. pSjEA devoid of proteins by proteinases treatment was also capable of inhibiting IgG bindings with pSjEA at dose-dependent fashion. A representative competitive inhibition curve on IgGs in infected sera was shown on panel 1 (mouse) and panel 3 (human) using untreated pSjEA as positive control for inhibition assay and OVA as negative control. A summary on calculated percentage of inhibition from infected mice (n = 5) or infected humans (n = 7) by enzyme-treated pSjEA was shown at panel 2 and panel 4. E. Con A, WGA and anti-Lewisx antibody staining of mock- and periodate-treated SjEA. Anti-GST was used as loading control. t test was used to analyze the significance of percent inhibition with *** as p < 0.001.