Figure 1From: Larvicidal activity and possible mode of action of four flavonoids and two fatty acids identified in Millettia pinnata seed toward three mosquito speciesIsolation procedures of larvicidal principles. The Millettia pinnata seed methanol extract was sequentially partitioned into hexane-, chloroform-, ethyl acetate-, butanol-, and water-soluble portions. For isolation of active principles from the chloroform-soluble fraction (A) and hexane-soluble fraction (B), 50Â mg/L of each M. pinnata seed-derived material was tested in a direct-contact mortality bioassay toward third instar larvae from Culex pipiens pallens and Aedes aegypti.Back to article page