Wolbachia density in wMel.F mosquito salivary glands. wMel.F mosquitoes fed once and twice on human bloodmeals (Fed 1x and Fed 2x) prior to being challenged with DENV-2 did not have a significant change in Wolbachia density in both replicates A and B compared to the Unfed controls which were not blood fed. Y-axis shows ratio of wsp/Rps17. Letters represent distinct statistical group. Error bars are standard error of the mean of 11–19 salivary glands.