Relative expression of RproIR76b in female and male antennae of R. prolixus using different normalization approaches. Two normalization approaches were used: applying the best normalization factor (left) and the worst potential reference gene (right). (A) Effect of nutrition in antennae of female larvae; (B) Effect of nutrition in antennae of female adults; (C) Effect of imaginal moulting in female antennae; (D) Effect of adult maturation in antennae of female adults; (E) Effect of nutrition in antennae of male larvae; (F) Effect of nutrition in antennae of male adults; (G) Effect of imaginal moulting in male antennae; and (H) Effect of adult maturation in antennae of male adults. Significant differences were calculated by using a two-tailed t-test for pair-wise comparisons. Asterisks denote statistically significant differences, * p <0.01; ** p < 0.01 and *** p < 0.0001. Error bars represent the standard error generated from 6 replicates per condition. L: larvae; A: adult; n.s.: non-significant difference.