Figure 1
From: Successful capture of Toxocara canis larva antigens from human serum samples
Determination of the optimal 1E4G4C2 MoAb concentration for the ELISA. 96-well polystyrene plate was coated with 0.0, 1.0, 2.5, 5.0, 10, 20 and 40 μg/mL of MoAb; non-specific binding sites were blocked with 1% skimmed milk; anti-mouse IgG-HRP conjugate was added at a 1:8,000 dilution and the reaction was revealed with chromogen/substrate solution. The results are shown as the arithmetic media ± standard deviation from three triplicate assays.