Fig. 1

a. Expression profile clustering of Toxoplasma gondii CDPK genes during host cell invasion. Extracellular: Harvested from outside the HFF cell. Intracellular_0hr: Harvested from inside the HFF cell, shortly after invasion; Intracellular_2hr: Harvested from inside the HFF cell, 2 h after invasion. Two biological replicates were performed each experiment. The colored bar representing the relative signal intensity values is shown at the top of the chart. Hierarchical clustering was used in data analysis. b. Expression profile clustering of Toxoplasma gondii CDPK genes across cell cycle expression profiles in HFF cell. h: the time post-thymidine release. Two biological replicates were performed each experiment. The colored bar representing the relative signal intensity values is shown at the top of the chart. Hierarchical clustering was used in data analysis. c. Expression profile clustering of Toxoplasma gondii CDPK genes across oocyst, tachyzoite and bradyzoite developmental stages. Oocysts were harvested from kitten feces and purified for RNA extraction at Day 0 (unsporulated), Day 4 post-induction of sporulation (mid-sporulation) and Day 10 post-induction of sporulation. In vitro, 2 dpi tachyzoite, 4 and 8 dpi bradyzoite samples are from separately infected cultures of HFF cell. In vivo, 21 dpi bradyzoite cysts harvested from the brains of mice that were infected with oocysts. 2 biological replicates each. The colored bar representing the relative signal intensity values is shown at the top of the chart. Hierarchical clustering was used in data analysis