Fig. 2
From: Molecular insights into a tetraspanin in the hydatid tapeworm Echinococcus granulosus
Immune recognition of recombinant and native Eg-TSP1 by Western blot. Lane 1, SDS-PAGE of purified rEg-TSP1 after dialysis and concentration; Lane 2 and lane 3, immune recognition of rEg-TSP1 using sera from E. granulosus infected mice (Lane 2) and healthy control (Lane 3); Lane 4 and lane 5, western blot analysis using recombinant Eg-TSP1 (Lane 4) and crude extracts of protoscolexes (Lane 5) against the purified rabbit anti-rEg-TSP1 IgG. A single band was detected in each lane with molecular weight of ~32Â kDa for recombinant Eg-TSP1 (partial sequence plus ~20Â kDa fusion peptide) and ~29Â kDa for native Eg-TSP1, respectively