Serological and molecular diagnostic surveys combined with examining hematological profiles suggests increased levels of infection and hematological response of cattle to babesiosis infections compared to native buffaloes in Egypt

Table 1 Sequences of the oligonucleotide primers used in the PCR assays and expected PCR product sizes

Parasite	Primer name	Sequence	Product Size	Source gene name and reference
Babesia bovis	BoF	5’ ATTGGCATCTGGGCTAAGTG 3’	823 bp	B. bovis Rhoptry associated protein related antigen (rra) gene
	BoR	5’ CAGCCCATTTCACAGGTTTT 3’	823 bp
	BoNF	5’ TGTTCCTGAGCCGCTATCTT 3’	387 bp
	BoNR	5’ CAGCCCATTTCACAGGTTTT 3’	387 bp
Babesia bigemina	BiF	5’ ATGATTCACTACGCTTGCCTC 3’	600 bp	B. bigemina Rhoptry associated protein (rap-1c) gene
	BiR	5’ GTCTTGTAGTATATGGCGGTCATGTAG 3’	600 bp
	BiNF	5’ TCTCGAAGACAGCGAACAGA 3’	236 bp
	BiNR	5’ GTGAAGCTGGTAGGGGTCAG 3’	236 bp

The primer sets used for the primary reaction were: BoF and BoR for the amplification of the rra B. bovis gene, and BiF and BiR for the amplification of the rap-1c B. bigemina gene. The primer sets used for semi-nested / nested PCR reaction were: BoNF and BoNR for amplification of the rra gene of B. bovis and BiNF and BiNR amplification of the rap-1c gene of B. bigemina

ISSN: 1756-3305