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Fig. 3 | Parasites & Vectors

Fig. 3

From: Identification and functional characterization of a novel arginine/ornithine transporter, a member of a cationic amino acid transporter subfamily in the Trypanosoma cruzi genome

Fig. 3

Trans-stimulation in Xenopus laevis oocytes expressing TcCAT1.1. Oocytes were pre-loaded overnight with 1 mM arginine. Subsequently, uptake assays were performed by incubating oocytes expressing TcCAT1.1 with 100 μM [3H]-arginine. a TcCAT1.1-injected oocytes that were pre-loaded with arginine (●); TcCAT1.1-injected oocytes that were not pre-loaded with arginine (▼); and water-injected oocytes, used as controls (○). b Efflux of [3H]-arginine or arginine sub-products from oocytes injected with TcCAT1.1 cRNA (●) and from control oocytes injected with water (○). Oocytes were pre-loaded for 6 h with 1 μM [3H]-arginine in ND96 buffer. c Effect of proton uncouplers on [3H]-arginine uptake in pre-loaded oocytes expressing TcCAT1.1

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Parasites & Vectors

ISSN: 1756-3305

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