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Fig. 5 | Parasites & Vectors

Fig. 5

From: Identification and functional characterization of a novel arginine/ornithine transporter, a member of a cationic amino acid transporter subfamily in the Trypanosoma cruzi genome

Fig. 5

Localization of EGFP-TcCAT1.1 in Trypanosoma cruzi. Stable transfectants of Dm28c over-expressing EGFP-TcCAT1.1 were cloned and maintained in 500 μg/ml of G418. Expression was evaluated by confocal microscopy. Panel (a) shows the intracellular distribution of EGFP-TcCAT1.1 at the anterior regions of the parasite (arrows). Panel (b) shows additional sites of reduced expression following incubation with anti-GFP, including the epimastigote surface (arrow head). Panels (c) and (d) show endocytosis with transferrin-Alexa Fluor 546 (small arrow) after 1 min in ammonium chloride-treated epimastigotes (c) and after 15 min (d). Panels (e) and (f) show the results of labeling with anti-RAB7 (small arrow) to evaluate co-localization of EGFP-TcCAT1.1 (arrow) with the Golgi complex. The left column shows differential interference contrast microscopy, the central column depicts EGFP-TcCAT1.1 expression (green), and the right column shows DAPI labeling of the nucleus and kinetoplast (blue), anti-RAB7, and transferrin-Alexa Fluor 546 tracer (red). N, nucleus; K, kinetoplast; FP, flagellar pocket

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Parasites & Vectors

ISSN: 1756-3305

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