Table 1 Primer sequences used for RT-PCR and RACE
From: Identification of genes associated with blood feeding in the cat flea, Ctenocephalides felis
Transcript ID | ||
|---|---|---|
RT-PCR | Forward Primer | Reverse Primer |
B2 | tgctctcatcaaagtttctagtgc | ccagacgaaagacgtgacaccaac |
S5 | acccagtggctcgctccgcttatg | gctaacataggcagacaagccac |
S16 | acgacgtcgaacgttttgtgatgc | gccttgcaaatttcaccaccct |
B43 | aacctaaatctgatggcagtgatg | cacaattttgtatctgagctttcc |
S49 | actgttctatccctggtgtcaatg | gacaagaaccattcttgaatcctg |
B52 | catgggtggaatgatattggttac | gttgcctaataaatgctgtgtcag |
S58 | ccatctgtagcctacgactatgtc | agcgctcacgtagtcagcaacaa |
S61 | atgcacatatcccaatatggatac | gtttcctaagaacacctttgcaa |
B68 | agtgaccaccacttcctatgcaac | gtaactggagtggaaacaacattg |
RACE | 5’ RACE Primer | 3’ RACE Primer |
B2 | gcactagaaactttgatgagagca | gttggtgtcacgtctttcgtctgg |
S5 | cataagcggagcgagccactgggt | gtggcttgtctgcctatgttagc |
S16 | gcatcacaaaacgttcgacgtcgt | agggtggtgaaatttgcaaggc |
B43 | catcactgccatcagatttaggtt | ggaaagctcagatacaaaattgtg |
S49 | cattgacaccagggatagaacagt | caggattcaagaatggttcttgtc |
B52 | gtaaccaatatcattccacccatg | ctgacacagcatttattaggcaac |
S58 | gacatagtcgtaggctacagatgg | ttgttgctgactacgtgagcgct |
S61 | gtatccatattgggatatgtgcat | ttgcaaaggtgttcttaggaaac |
B68 | gttgcataggaagtggtggtcact | caatgttgtttccactccagttac |