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Fig. 3 | Parasites & Vectors

Fig. 3

From: Autophagic digestion of Leishmania major by host macrophages is associated with differential expression of BNIP3, CTSE, and the miRNAs miR-101c, miR-129, and miR-210

Fig. 3

Ultrastructural investigation of autophagy induction in L. m.-infected RAW 264.7 macrophages with TEM. Methods: RAW 264.7 macrophages were infected with L. m. promastigotes for (d, g, j) 0.5 h and (e, f, h, i, k, l) 24 h. ac Uninfected BMDM were incubated for the same amount of time in RPMI medium. All macrophages were subjected to TEM analysis. Results: Autophagic phenotypes characterized by (df) a strong vacuolization, (g, h) the presence of MLS, (l) autophagosomes, and (i) phagophores were observed in L. m.-infected RAW 264.7 macrophages 0.5 h p.i. and 24 h p.i. compared to uninfected controls. Details in images (gl) were magnified from images (df) from a total section of L. m.-infected BMDM (red squares = MLS in g and h, red circle = phagophore in i, black circle = autophagosome in l, black squares = intracellular parasites in j and k). N = nucleus of macrophage, NP = nucleus of parasite, P = parasite

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