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Fig. 1 | Parasites & Vectors

Fig. 1

From: Two COWP-like cysteine rich proteins from Eimeria nieschulzi (coccidia, apicomplexa) are expressed during sporulation and involved in the sporocyst wall formation

Fig. 1

Schematic drawing of the arrangements and content of expression cassette. EnOWP reporter cassettes are head-to-head orientated to the chimeric selectable marker (dhfr/ts-yfp) Etact5′: E. tenella actin promoter, dhfr/tsm2m3: encodes a pyrimethamine resistant dihydrofolate reductase-thymidilate synthase [25], yfp: yellow fluorescent protein; 3′: 3′flanking region of E.tenella actin gene. Construct 1: pEnOWP2pro-mC encodes the chimeric selectable marker and a mCherry gene under control of the EnOWP2-promoter; Construct 2: pEnOWP2-mC encodes the chimeric selectable marker and a mCherry gene fused to the full length genomic EnOWP2 controlled by the authentic promoter. Construct 3: pEnOWP2Exon1-mC encodes the chimeric selectable marker and a mCherry gene fused with the Exon1 of EnOWP2 controlled by the authentic promoter; Construct 4: pEnOWP6-mC encodes the chimeric selectable marker and a mCherry gene fused to the full length genomic EnOWP6 controlled by the authentic promoter

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Parasites & Vectors

ISSN: 1756-3305

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