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Fig. 9 | Parasites & Vectors

Fig. 9

From: Two COWP-like cysteine rich proteins from Eimeria nieschulzi (coccidia, apicomplexa) are expressed during sporulation and involved in the sporocyst wall formation

Fig. 9

Expression pattern of the EnOWP6-mCherry reporter during sporulation (construct 4) Bar 5 μm. a: Freshly harvested oocyst with cytoplasmatic YFP but absent mCherry signal. b: Oocyst after 6 h sporulation period with extracytoplasmatic signal and cytoplasmatic YFP signals. c: Comparison between transgenic oocyst with mCherry and YFP signal and wild type oocyst with only UV autofluorescence after 19 h sporulation period. Transgenic oocyst with cytoplasmatic and nuclear YFP signal, indicating first meiotic division, and extracytoplasmatic mCherry signals as well as a punctuated mCherry signals in the circumplasm. d: Transgenic oocyst after 20 h sporulation period with extracytoplasmatic mCherry and cytoplasmatic and nuclear YFP signal during first meiotic division. e: Oocyst after sporoblast formation (27 h sporulation period), the sporoblast with YFP signals is surrounded by extracytoplasmatic mCherry signals. f: Oocyst during sporozoite and sporocyst formation. The mCherry signals accumulate at the boundary of the sporoblast, probably involved in the formation emerging sporocyst wall, nuclear YFP signal within the sporoblast are indicating a mitotic process. Sporulation period 27 h g: Sporulated oocyst after 2d sporulation period. The red mCherry signals are mainly distributed to the sporocyst wall and are reduced in the circumplasm. Scale bar 5 μm. TL: transmitted light; YFP: YFP fluorescence; mCherry: mCherry fluorescence; MCF (multichannel fluorescence) overlay of all fluorescent signals in the row

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Parasites & Vectors

ISSN: 1756-3305

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