Table 1 Trypsin-like serine peptidases identified by mass spectrometry in the midgut of Culex quinquefasciatus females fed on sugar

Trypsin 4 OS = Culex quinquefasciatus GN = CpipJ_CPIJ017414

B0XCW2_CULQU

28 kDa

3

13

18

6

29

28

(R)VGSSYDYQGGTVIDVAGMTIHPR(Y)

35.21

(R)VGSSYDYQGGTVIDVAGMTIHPR(Y)

(K)DFDFALLR(L)

52.94

(K)DFDFALLR(L)

(K)GCAQPDYYGVYADVEK(A)

39.12

(K)GCAQPDYYGVYADVEK(A)

(K)NMLCAGYDEGLR(D)

(R)LSWIGVR(V)

(R)ENYAESR(L)

Trypsin 7 OS = Culex quinquefasciatus GN = CpipJ_CPIJ017964

B0XES8_CULQU

27 kDa

4

14

18

5

25

18

(R)GGQLIAVTR(K)

53.31

(R)GGQLIAVTR(K)

(R)DYALLNLAK(S)

50.34

(R)DYALLNLAK(S)

(R)AVDVPIADHDR(C)

24.69

(R)AVDVPIADHDR(C)

(K)DACLGDSGGPLTCSGK(V)

49.46

(K)DACLGDSGGPLTCSGK(V)

(F)M*LCAGYDAGGK(D)

Trypsin-5 OS = Culex quinquefasciatus GN = CpipJ_CPIJ015103

B0X667_CULQU

30 kDa

3

7

16

3

14

16

(K)IIGGFPAEQGDTLHQVSIR(F)

35.64

(K)IIGGFPAEQGDTLHQVSIR(F)

(K)GCGLAAYPGIYSDVAYYR(G)

29.61

(K)GCGLAAYPGIYSDVAYYR(G)

(R)GWIDSCLAGK(C)

31.7

(R)GWIDSCLAGK(C)

Trypsin-1 OS = Culex quinquefasciatus GN = CpipJ_CPIJ007079

B0WIS4_CULQU

29 kDa

3

6

18

6

10

34

(R)IVGGFEISIADAPHQVSLQSR(G)

51.51

(R)IVGGFEISIADAPHQVSLQSR(G)

(K)HASGGSVISIK(R)

26.21

(K)HASGGSVISIK(R)

(R)AAYVPAYNQNQCNSAYAR(Y)

29.15

(R)AAYVPAYNQNQCNSAYAR(Y)

(K)DACQGDSGGPLVADGK(L)

33.42

(R)NTIDYDYSLLELK(S)

(R)GSHICGGSIISPK(W)

(K)WILTAAHCTDGASVSNLR(I)

Trypsin 2 OS = Culex quinquefasciatus GN = CpipJ_CPIJ005273

B0WE94_CULQU

28 kDa

2

2

12

5

13

30

(R)LEFGHAVQPVDLVR(D)

19.14

(R)LEFGHAVQPVDLVR(D)

(R)DEPADESQSLVSGWGDTR(S)

27.7

(R)DEPADESQSLVSGWGDTR(S)

(R)WVLTAAHCTENTDAGIYSVR(V)

(R)GVLVPLVNR(E)

(K)LGMPVTESMICAGFAK(E)

Serine protease1/2 OS = Culex quinquefasciatus GN = CpipJ_CPIJ003826 PE = 3 SV = 1

B0W9S9_CULQU

30 kDa

2

3

18

2

3

10

(R)TGETFVDNQATVSGFGR(T)

35.91

(R)TGETFVDNQATVSGFGR(T)

(R)TVDGGPVSPTK(N)

35.12

(R)TVDGGPVSPTK(N)

Serine protease SP24D OS = Culex quinquefasciatus GN = CpipJ_CPIJ015368

B0X870_CULQU

27 kDa

1

1

10

1

3

10

(K)LGESIEYDELSQPIALYEGDDLPK(D)

34.98

(K)LGESIEYDELSQPIALYEGDDLPK(D)

Cationic trypsin OS = Culex quinquefasciatus GN = CpipJ_CPIJ011378

B0WW44_CULQU

26 kDa

1

1

8

(R)IVVHPQYAEGNLANDIAVIR(V)

32.92