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Table 2 General characteristics of Culex quinquefasciatus trypsin-like serine peptidases identified by mass spectrometry ∆

From: In-depth characterization of trypsin-like serine peptidases in the midgut of the sugar fed Culex quinquefasciatus

Uniprot accession number Protein name Active sitetriad position Cysteine pair residues Residues confering substrate specificity Protein size (aa) Activation site Conserved regions
Precursor Mature LTAAHC DIAL GDSGGP
B0WIS4 Trypsin 1 His88, Asp133, Ser229 73-89, 198-214, 225-249 Asp223, Gly246,Gly256 274 226 YR^IVGG LTAAHC DYSL GDSGGP
B0WE94 Trypsin 2 His75, Asp120, Ser216 60-76, 183-200, 212-236 Asp210, Gly234,Gly244 261 226 GK^IVGG LTAAHC DFCL GDSGGP
B0XCW2 Trypsin 4 His70, Asp116, Ser213 151-219, 181-198, 209-233 Asp207, Gly230,Gly240 258 233 FH^IVNG LTAAHL DFAL GDSGGP
B0X667 Trypsin 5 His73, Asp127, Ser221 58-74, 160-227, 192-208, 217-241 Asp215, Gly238,Gly248 293 268 PK^IIGG LTAAHC DIAL GDSGGP
B0XES8 Trypsin 7 His67, Asp112, Ser207 52-68, 146-213, 176-192, 203-227 Asp201, Gly224,Gly234 252 229 SR^IVNG LTAGHC DYAL GDSGGP
B0X870 SP24D His63, Asp109, Ser195 48-64, 172-181, 191-216 ? 240 217 RR^IFGG LTAAHC DIAL GDSGGP
B0W9S9 Serine protease 1/2 His92, Asp135, Ser233 77-93, 202-217, 229-259 ? 283 235 SR^IVNG LTAAHC DIGL GDSGGP
B0WW44 Cationic trypsin His69, Asp114, Ser202 54-70 ? 244 216 GR^VVGG LTAGHC DIAV YDGGSP
  1. ∆ Extracted after CLUSTAL Omega aligment analysis
  2. (aa) total number of amino acid residues
  3. ? = other residues different than DGG
  4. ^ = Indicates the clivage site for zymogen activation