Fig. 4
From: Nitric oxide maintains cell survival of Trichomonas vaginalis upon iron depletion
NO is important for cell survival in iron-deficient T. vaginalis. The growth of iron-deficient cells after treatment with different drugs was monitored every three hours using the trypan blue exclusion assay. The solid curve (circle) reveals the cell density of iron-deficient (DIP, 180 μM) cells; the dashed curve (square) reveals the cell density of MG132 (10 μM)-treated iron-deficient cells; the dot-dashed curve (triangle) reveals the cell density of MG132 and arginine (5 mM) co-treated iron-deficient cells. The data are presented as the mean ± SD of three independent experiments