Fig. 4

Profiles of antigens recognized by domestic duck IgY in homogenates of cercariae and schistosomula on Western blots. Reactions with sera from domestic ducks exposed to a single dose of 1500 cercariae (group SD) are shown as an illustrative example of the reactions. Cercarial homogenate = lanes 1–5 and 10–14. Homogenate of schistosomula = lanes 6–9 and 15–19. Lane M - markers of MW; lanes 1, 2, 3, 4, 5 - sera of an infected duck taken on days 10, 15, 20, 30 and 40 after initiation of the experiment; lanes 6, 7, 8, 9 - sera of a non-infected duck on days 10, 20, 30 and 40 days; lanes 10, 11, 12, 13 and 14 - sera of an infected duck on days 0, 10, 20, 30 and 40 pi; lanes 15, 16, 17, 18 and 19 - sera of an infected duck on days 0, 10, 20, 30 and 40 pi. Red arrowheads - cercarial antigens 32 and 19 kDa specifically recognized by IgY; blue arrowheads – antigens of schistosomula 64, 54, 44, 39 and 18 kDa specifically recognized by IgY; violet arrowheads - antigens 50 and 47 kDa commonly recognized by IgY in both cercariae and schistosomula