Fig. 1

Gene structure and expression of A. stephensi p38 MAPK. (a) The A. stephensi p38 (AsP38) MAPK gene includes 7 exons (black boxes; length in base pairs (bp) indicated), 6 introns, and spans 17,303 bp. (b) Total RNA was isolated and converted to cDNA from 30 dissected midguts from non-blood fed female A. stephensi. AsP38 MAPK-specific primers were used to amplify cDNA by conventional PCR; molecular standards (bp) are shown on left