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Table 1 Metabolic activity of human PBMCs

From: Phytoecdysteroids as modulators of the Toxoplasma gondii growth rate in human and mouse cells

   Metabolic activity [%] of human PBMCs
24 h 48 h 72 h
Cmpd. C [μg/mL] MTT MTT [3H]-thymidine
ConA 2.5 91.62 ± 19.97 115.67 ± 24.71 *189.69 ± 42.55 *926.57 ± 169.21
α-ecdysone 2 69.76 ± 32.29 84.63 ± 25.67 76.04 ± 26.90 92.27 ± 18.25
10 89.51 ± 22.83 93.81 ± 30.35 85.59 ± 24.40 94.19 ± 19.46
20 91.97 ± 25.74 94.73 ± 23.05 97.07 ± 26.87 94.08 ± 26.97
20-hydroxy ecdysone 2 97.32 ± 20.23 97.69 ± 29.34 105.03 ± 22.52 99.21 ± 21.45
20 100.92 ± 15.48 102.92 ± 27.78 107.60 ± 20.78 100.13 ± 27.84
100 95.68 ± 15.57 102.05 ± 25.34 106.62 ± 22.18 92.71 ± 26.51
sulfadiazine 2 95.74 ± 12.74 103.15 ± 27.05 107.30 ± 26.87 91.24 ± 22.48
20 98.37 ± 18.35 104.37 ± 20.69 107.00 ± 26.21 96.23 ± 24.57
100 100.63 ± 19.82 106.38 ± 26.83 104.51 ± 21.83 89.68 ± 28.18
  1. PBMCs isolated from individuals (N = 61) were tested in the presence of phytoecdysteroids and sulfadiazine in the concentration range between 2 and 100 μg/mL ± SD at the incubation time from 24 to 72 h. The statistically significant differences (p < 0.05) were labeled with an asterisk (*); to calculate the decrease in the metabolic rate compared to the untreated blank, the following equation was used: viability measured using the MTT assay [%] = 100 × sample OD570 (the mean value of the measured optical density of the test samples) / blank OD570 (the mean value of the measured optical density of the untreated cells); using the [3H]-thymidine test: 100 × mean cpm incorporated into treated cells / mean cpm incorporated into cells cultured without phytoecdysteroids