Phytoecdysteroids as modulators of the Toxoplasma gondii growth rate in human and mouse cells

Parasites & Vectors

Table 1 Metabolic activity of human PBMCs

		Metabolic activity [%] of human PBMCs
		24 h	48 h	72 h
Cmpd.	C [μg/mL]	MTT		MTT	[3H]-thymidine
ConA	2.5	91.62 ± 19.97	115.67 ± 24.71	^*189.69 ± 42.55	^*926.57 ± 169.21
α-ecdysone	2	69.76 ± 32.29	84.63 ± 25.67	76.04 ± 26.90	92.27 ± 18.25
	10	89.51 ± 22.83	93.81 ± 30.35	85.59 ± 24.40	94.19 ± 19.46
	20	91.97 ± 25.74	94.73 ± 23.05	97.07 ± 26.87	94.08 ± 26.97
20-hydroxy ecdysone	2	97.32 ± 20.23	97.69 ± 29.34	105.03 ± 22.52	99.21 ± 21.45
	20	100.92 ± 15.48	102.92 ± 27.78	107.60 ± 20.78	100.13 ± 27.84
	100	95.68 ± 15.57	102.05 ± 25.34	106.62 ± 22.18	92.71 ± 26.51
sulfadiazine	2	95.74 ± 12.74	103.15 ± 27.05	107.30 ± 26.87	91.24 ± 22.48
	20	98.37 ± 18.35	104.37 ± 20.69	107.00 ± 26.21	96.23 ± 24.57
	100	100.63 ± 19.82	106.38 ± 26.83	104.51 ± 21.83	89.68 ± 28.18

PBMCs isolated from individuals (N = 61) were tested in the presence of phytoecdysteroids and sulfadiazine in the concentration range between 2 and 100 μg/mL ± SD at the incubation time from 24 to 72 h. The statistically significant differences (p < 0.05) were labeled with an asterisk (*); to calculate the decrease in the metabolic rate compared to the untreated blank, the following equation was used: viability measured using the MTT assay [%] = 100 × sample OD₅₇₀ (the mean value of the measured optical density of the test samples) / blank OD₅₇₀ (the mean value of the measured optical density of the untreated cells); using the [³H]-thymidine test: 100 × mean cpm incorporated into treated cells / mean cpm incorporated into cells cultured without phytoecdysteroids

ISSN: 1756-3305