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Table 3 Effect of the studied phytoecdysteroids on the intensity of RH T. gondii proliferation

From: Phytoecdysteroids as modulators of the Toxoplasma gondii growth rate in human and mouse cells

Cmpd.

C [μg/mL]

Proliferation of parasites [%] in mouse splenocytes

BALB/c

C57BL/6

Toxo-positive (N = 10)

Toxo-negative (N = 14)

Toxo-negative (N = 10)

α-ecdysone

2

89.29 ± 5.90 ↓

67.77 ± 5.05*

89.69 ± 2.54 ↓

10

91.17 ± 2.89 ↓ 10 %

75.69 ± 2.34* ↓ 39 %

99.75 ± 2.57 ↓ 13 %

20

99.29 ± 4.14 ↓

106.63 ± 2.67*

102.58 ± 1.85 ↓

20-hydroxy ecdysone

2

88.65 ± 2.55

80.81 ± 3.35*

99.30 ± 2.55

20

88.82 ± 3.11

109.28 ± 2.11*

103.73 ± 3.94

100

90.02 ± 0.52

92.05 ± 2.34

97.76 ± 1.85

sulfadiazine

2

92.91 ± 5.11

78.34 ± 5.14*

85.79 ± 1.55*

20

94.18 ± 2.35

70.87 ± 3.11*

74.65 ± 2.48*

100

81.48 ± 1.52

65.32 ± 3.09*

62.31 ± 2.88*

ConA

2.5

192.60 ± 9.65*

151.48 ± 8.97*

191.62 ± 9.97*

  1. Parasite proliferation was tested on splenocytes obtained from BALB/c mice previously infected with DX T. gondii (Toxo-positive), BALB/c and C57BL/6 uninfected (Toxo-negative) mice, using [3H] uracil incorporation. The statistically significant differences (p < 0.05) were labeled with an asterisk (*); ↓ indicates an increasing pool of protozoans with the increasing concentration of phytoecdysteroids in the splenocyte cultures