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Fig. 2 | Parasites & Vectors

Fig. 2

From: Nitric oxide stimulates early egress of Toxoplasma gondii tachyzoites from human foreskin fibroblast cells

Fig. 2

Exogenous NO-induced egress of T. gondii from HFFs was dependent on calcium flux of the parasite. Tachyzoite-infected HFFs were pre-treated with BAPTA-AM (a) or BAPTA (b) to chelate calcium in the parasite cytosol or host cells, respectively, before NO-induced egress assay as described in the Methods section. Egressed parasites were suspended with 500 μL of DMEM, and 20 μL suspensions were analysed by flow cytometry. The data in the figure show the means ± SEMs of four replicates and represent three independent experiments, **P < 0.01

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