Table 1 Primer sequences used for the qPCR assay and probes and PNA sequences used for the Reverse line blotting method in this study
Name | Target organism | Position | Sequence |
|---|---|---|---|
12Sov | Sheep (Ovis aries) | Forward | CCAGCCTTCCTGTTAACTTTCAATAGACT |
Reverse | TTTAGTCCTGTGTGATTCGAAGGGCG | ||
12Sgg | Chicken (Gallus gallus) | Forward | CTCGCTAATAAGACAGGTCAAGGTA |
Reverse | TAGGGGGTATGATCTCACTTTACTG | ||
Chicken probe | Chicken (Gallus gallus) | - | Amino-ACCTCCCATCACACATGT |
Sheep probe | Sheep (Ovis aries) | - | Amino-AAATAATTATAAAAACAAAATTATTC |
PNA clamp | Human (Homo sapiens) | Reverse | H-GTGTTCTGGCGAGCAGTT-NH2 |