Fig. 1

CHIKV nsP3 forms granules in insect cells. a Schematic representation of CHIKV nsP3 variants used in this study. Representing either the full length protein N-terminally fused to EGFP (nsP3), nsP3 isoforms with adapted C-terminal amino acids (nsP3, nsP3-DDEL and nsP3-dUAG) or truncated nsP3 variants (nsP3.2, 3.7, 3.8 and 3.10). Asterisk indicates opal stop codon between nsP3 and nsP4. b Intracellular distribution of wild type CHIKV nsP3 in cultured insect and mammalian cells. Mosquito and lepidopteran cells were transfected with plasmids that express the nsP3 variants from OpIE2 insect promoters. In mammalian cells expression of the nsP3 variants was driven by a cytomegalovirus promoter. c Intracellular distribution of nsP3-DDEL and nsP3-dAUG in insect cells. d Intracellular distribution of the truncated nsP3 variants