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Table 5 Comparison of fragment sizes obtained when bovine Cryptosporidium parvum DNA was processed in two different laboratories

From: Development of a framework for genotyping bovine-derived Cryptosporidium parvum, using a multilocus fragment typing tool

Marker MRI allele MRI UoG
Size (bp) Prevalence (n = 118) Size (bp)a Prevalence (n = 211)
MM5 1 262 78 % 260 69 %
2 235 22 % 233 29 %
NA 288 0 % 287 0.5 %
MM18 1 288 73 % 290 56 %
2 294 22 % 296 2 %
3 318 4 % NA 0 %
NA 299 0 % 302 0.5 %
MM19 1 298 53 % 299 38 %
2 304 4 % 305 4 %
3 292 33 % 293 55 %
4 316 2 % NA 0 %
5 270 2 % 269 0.5 %
6 253 3 % NA 0 %
7 281 3 % 281 0.5 %
NA 310 0 % 311 1 %
TP14 1 296 78 % 297 60 %
2 305 22 % 306 40 %
MS1 1 361 100 % 362 99 %
NA 327 0 % 326 0.5 %
NA 384 0 % 386 0.5 %
MS9 1 455 100 % 444 96 %
NA 443 0 % 432 1.5 %
NA 461 0 % 450 2 %
  1. DNA was prepared and initially sized at the University of Glasgow (UoG), then amplified and sized at Moredun Research Institute (MRI). In addition the prevalence of each allele is given for the two studies. Only primary peaks were used to assign allele number
  2. aAllele sizes are the binned Genescan results